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Correlation between clinical findings, mast cell count and interleukin 31 immunostaining in the skin of dogs with atopic dermatitis Ciência Rural
Gonçalves,Bárbara Hess Rodrigues; Matos,Bruna Dantas; Faleiro,Mariana Batista Rodrigues; Arnhold,Emmanuel; Matos,Moema Pacheco Chediak; Santin,Ana Paula Iglesias; Moura,Veridiana Maria Brianezi Dignani de.
ABSTRACT: In this study the correlation between the clinical score, mast cell count and interleukin 31 (IL-31) immunostaining in the skin of dogs with atopic dermatitis was determined. A total of 31 dogs of different breeds, from one to eight years of age, were chosen for the study. The 20 females and 11 males were categorized based on the CADESI-4 system, as having discrete, moderate or marked atopic dermatitis. Skin samples were collected from the axillary and interdigital regions and stained with hematoxylin and eosin for cytohistomorphological analyses and toluidine blue to evaluate the mast cell counts, and immunohistochemistry for the IL-31 immunostaining. Animals revealing higher atopic dermatitis scores had greater numbers of mast cells and IL-31...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine allergy; Pro-inflammatory cytokine; Toluidine blue.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900600
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Morphological study on the encystment of the ciliated protozoan Colpoda cucullus OAK
Watoh, Tomoko; Sekida, Satoko; Yamamoto, Keisuke; Kida, Akemi; Matsuoka, Tatsuomi.
Morphological changes during resting cyst formation (encystment) of Colpoda cucullus were examined. At 1 hr after encystment induction, a number of vesicles containing the electron-dense and toluidine blue (TB) positive materials were fused with large vacuoles to excrete the contents, and the vacuoles opened to the extracellular space to excrete. In this stage, the rounded precystic cells were surrounded by the first-synthesized cyst wall layer (L1 layer), and the cilia were resorbed inside the L1 layer. Thereafter (1-2 hr), a secondary layer (L2 layer) was formed and subsequently a deeply TB stained substance was diffused into the space between the plasma membrane and L2 layer. At 3-6 hr, numerous endoplasmic reticula (ER) associated with ribosomes...
Palavras-chave: Colpoda; Encystment; Cyst wall; Toluidine blue.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/148
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